Journal: Frontiers in Bioengineering and Biotechnology
Article Title: EP4 receptor stimulation in combination with core decompression therapy enhanced bone regeneration in a canine model of osteonecrosis of femoral head
doi: 10.3389/fbioe.2025.1622918
Figure Lengend Snippet: HE staining and immunohistochemistry of human femoral heads from patients with hip fracture (upper panels, 66 years old, female) and ONFH (lower panels, 73 years old, female). The black boxes are shown at higher magnification. Arrowheads indicate EP4-positive lining cells. HE, hematoxylin–eosin; ONFH, osteonecrosis of the femoral head.
Article Snippet: Immunohistochemical staining for EP4 was performed using a rabbit anti-EP4 antibody (1.0 mg/mL, NLS3890; Novus Biologicals, Centennial, CO, United States) or rabbit IgG (I-1000; Vector Laboratories, Newark, CA, United States) as a negative control.
Techniques: Staining, Immunohistochemistry